More than 650 people are taking part in this year’s Durrell Dash.
The two races, of either 6km or 13km start and finish at Jersey Zoo.
Thanks to Jersey Water, runners will be able to rehydrate along the Trinity route without the use of single-use plastic bottles.
Jersey Zoo stopped selling them last year in an effort to reduce packaging and waste.
Alexandra Shears from Durrell says money raised will support the zoo’s new butterfly and tortoise exhibit.
“Just recently we’ve opened our new ‘Butterfly Kaleidoscope’ which is a new exhibit in the zoo where visitors can walk through a stunning and tropical wonderland with free-flying butterflies and tortoises.
“We just opened that last weekend, but obviously with the opening of this new exhibit comes significant running costs, heating, lots of technology, new staff etc.”

Demolition of Victoria Marine Lake would be considered
17 schools create new 'Reduce, Reuse, Recycle' artwork
Senator Sir Mark Boleat appointed Planning and Regulation Minister
Trial begins of man accused of sexual assaults in St Helier
JSPCA seeks help to build new rural animals' shelter
Call to remain vigilant after spate of wildfires
Third heatwave ends, but more warm weather still to come
50 people evacuated from scene of fire in Portbail-sur-Mer