David Luiz, Mateo Kovacic and Emerson Palmieri are also absent from Maurizio Sarri’s 21-man squad.
Hazard has featured in all five of Chelsea’s Premier League matches this season, scoring three goals as Sarri’s side made a perfect start to the campaign.
The decision appears to have been made to allow the Belgian some rest ahead of a run of three games in seven days, in which they face West Ham away, live on Sky Sports, before a Carabao Cup tie against Liverpool and a Premier League match against the same opponents three days later.
Cesc Fabregas is included in the travelling party, despite having not featured in the league so far this season.
Meanwhile, midfielder Danny Drinkwater has not been registered in the Europa League squad for the group stages, due to competition rules about the number of homegrown players that must be in the team.
Ethan Ampadu, who signed a new five-year deal at the club on Wednesday, has been selected alongside fellow youth prospect Callum Hudson-Odoi.
The 21-man squad to face PAOK:
Kepa Arrizabalaga, Willy Caballero, Marcin Bulka, Toni Rudiger, Marcos Alonso, Cesc Fabregas, Jorginho, N’Golo Kante, Ross Barkley, Pedro, Ruben Loftus-Cheek, Victor Moses, Olivier Giroud, Callum Hudson-Odoi, Davide Zappacosta, Willian, Gary Cahill, Andreas Christensen, Cesar Azpilicueta, Alvaro Morata, Ethan Ampadu.