Worn by the world record holder during the 100m heats at London’s 2012 Olympic Games, the signed trainers are part of an extensive collection built up by an unnamed fan in Derby.
They were stolen during a burglary at his house in Linton, and have not yet been recovered.
The victim said: “The spikes are part of an extensive collection that I have built up over the last 10 years.
“There are only four or five pairs of spikes that have been signed from the London 2012 Olympics – they are absolutely irreplaceable.”
The white, red and blue Puma Evosprints were used during the 100m heats. Bolt went on to win the 100m Olympic title that year, as well as 200m title.
He retired in 2017.
A 35-year-old man has been charged in connection with theft from a Linton address on 10 June.
:: Anyone with information about the shoes should call police on 101 or Crimestoppers on 0800 555111.