All 22 other members of the squad trained in what was England’s first full session since their win over Tunisia.
Gareth Southgate’s side play Panama in their second Group G game on Sunday.
The England manager took England training on Thursday, despite the discomfort of a dislocated shoulder.
Southgate took a tumble while running alone near England’s World Cup camp in Repino and was treated in a nearby hospital.
There was no sign of the black sling he had been pictured wearing on Wednesday and Southgate led the session as normal, flanked by assistant Steve Holland.
Southgate appeared in good spirits and made little concession to his injury, keeping his right arm close to his body, but otherwise showing no outward signs of pain.
Alli, who had his injury scanned on Tuesday, said he will do “everything in my power” to reach full fitness for the fixture, having taken to Twitter to voice his frustration at the injury.
Fabian Delph was stripped and seemingly ready to come on when Alli first appeared to take the knock in the first half against Tunisia, but the 22-year-old was kept on until the 80th minute when he was replaced by Ruben Loftus-Cheek.
Southgate said Alli had felt he could carry on at half-time despite the knock, with England going in search of a winner having been drawing 1-1 at the break.